Journal article
Investigation of the endosperm-specific sucrose synthase promoter from rice using transient expression of reporter genes in guar seed tissue
We report the investigation of an endosperm-specific promoter from the rsus3 gene from rice (Oryza sativa). The promoter was characterized by deletion analysis and transient expression in guar (Cyamopsis tetragonoloba) seed-tissue. Transient expression was monitored by histochemical GUS assay, and quantitative dual reporter assays comprising firefly luciferase as a test reporter, and Renilla luciferase and GUS as reference reporters.
These revealed high expression levels of the reporter genes directed by the rsus3 promoter in guar endosperm. Specificity for this tissue in seeds was apparent from a virtual absence of reporter activity in guar cotyledons. Removal of a putative intron region only slightly raised the expression level, whereas duplication of the minimal promoter region, in a tandem-repeat rsus3 promoter construct, retained endosperm specificity in guar, and displayed three times the reporter activity observed with the single copy construct.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 2006 |
Pages: | 1035-1042 |
ISSN: | 1432203x and 07217714 |
Types: | Journal article |
DOI: | 10.1007/s00299-006-0158-x |
ORCIDs: | Rasmussen, Thomas Bruun |
Base Sequence Cyamopsis Dual reporter Endosperm Gene Expression Gene Expression Regulation, Plant Genes, Plant Genes, Reporter Glucosyltransferases Glucuronidase Guar Introns Luciferase Molecular Sequence Data Oryza Plants, Genetically Modified Promoter Regions, Genetic Rsus3 promoter Seeds Transient expression sucrose synthase