Journal article
Long-chain PUFA in Granulocytes, Mononuclear Cells, and RBC in Patients With Cystic Fibrosis: Relation to Liver Disease : Relation to Liver Disease
Background and Aim: Patients with cystic fibrosis (CF) have low levels of n-3 long-chain polyunsaturated fatty acids (n-3 LCPUFA) in plasma or red blood cells (RBC), as also seen in other chronic and acute liver diseases. The differences may be more pronounced in CF transmembrane conductance regulator protein (CFTR)-regulated tissues such as granulocytes, monocytes, and lymphocytes.
The aim of the present study was to investigate whether patients with CF-related liver disease have lower n-3 LCPUFA level than patients with CF without liver disease.Methods: Twenty patients with known CF-related liver disease were matched with 20 CF patients without. Blood samples were analysed for liver biochemistry and haematology.
Granulocytes, mononuclear cells, and RBC were separated by density gradient centrifugation, and fatty acid composition was measured by gas chromatography. Hepatic ultrasound was scored according to Williams et al. Hepatic transit time (HTT) was measured with the ultrasound contrast agent SonoVue.Results: No significant differences were seen in either n-6 or n-3 LCPUFAs in any cell line when the 2 groups were compared.
In a multiple regression analysis including HTT, age, Pseudomonas aeruginosa infection, diabetes mellitus, treatment with ursodeoxycholic acid, forced expiratory volume in 1 second (% of predicted value), and Williams' ultrasound scoring scale, only n-3 LCPUFA docosahexaenoic acid in mononuclear cell membranes was positively associated with HTT (P = 0.02).
The arachidonic acid/docosahexaenoic acid ratio within the mononuclear cells was negatively associated with both HTT (P = 0.003) and Williams' ultrasound scoring scale (P = 0.03). For RBC-LCPUFAs, no significant associations were seen.Conclusions: These findings indicate that in patients with CF, the degree of liver disease was negatively associated with LCPUFA n-3 levels in CFTR-expressing white blood cells but unrelated to those levels in CFTR-negative RBC.
| Language: | English |
|---|---|
| Year: | 2012 |
| Pages: | 76-81 |
| ISSN: | 02772116 and 15364801 |
| Types: | Journal article |
| DOI: | 10.1097/MPG.0b013e318249438c |
| ORCIDs: | 0000-0003-0449-0839 |
02506, Cytology - Animal 02508, Cytology - Human 03508, Genetics - Human 10066, Biochemistry studies - Lipids 10067, Biochemistry studies - Sterols and steroids 13002, Metabolism - General metabolism and metabolic pathways 13020, Metabolism - Metabolic disorders 14006, Digestive system - Pathology 15002, Blood - Blood and lymph studies 15004, Blood - Blood cell studies 16006, Respiratory system - Pathology 17002, Endocrine - General 17008, Endocrine - Pancreas 25000, Pediatrics 25503, Development and Embryology - Pathology 31000, Physiology and biochemistry of bacteria 34502, Immunology - General and methods 36002, Medical and clinical microbiology - Bacteriology Allied Medical Sciences CF transmembrane conductance regulator protein CFTR Clinical Endocrinology Gastroenterology Gram-Negative Aerobic Rods and Cocci Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Pseudomonadaceae [06508] Pseudomonas aeruginosa species pathogen Human Medicine, Medical Sciences Medical Genetics Metabolism PUFA Pediatrics Primates Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Humans, Mammals, Primates, Vertebrates) - Hominidae [86215] human common child, preadolescent child, adolescent host female Pseudomonas infection bacterial disease SDG 3 - Good Health and Well-being SonoVue 2551-62-4 cystic fibrosis Cystic Fibrosis (MeSH) respiratory system disease, genetic disease, congenital disease, digestive system disease density gradient centrifugation laboratory techniques diabetes mellitus Diabetes Mellitus (MeSH) endocrine disease/pancreas, metabolic disease docosahexaenoic acid 32839-18-2 gas chromatography laboratory techniques, chromatographic techniques granulocyte immune system, blood and lymphatics hepatic ultrasound clinical techniques, diagnostic techniques liver disease Liver Diseases (MeSH) digestive system disease lymphocyte immune system, blood and lymphatics monocyte immune system, blood and lymphatics mononuclear cell immune system, blood and lymphatics multiple regression analysis mathematical and computer techniques plasma blood and lymphatics red blood cell blood and lymphatics ursodeoxycholic acid 128-13-2
Adolescent Adult Alanine Transaminase Animals Arachidonic Acid Aspartate Aminotransferases Bilirubin Child Child, Preschool Cystic Fibrosis Diet Docosahexaenoic Acids Eicosapentaenoic Acid Erythrocytes Fatty Acids, Omega-3 Fatty Acids, Unsaturated Female Fishes Granulocytes Humans Leukocytes, Mononuclear Liver Diseases Male Oleic Acid Questionnaires Surveys and Questionnaires Ultrasonography Young Adult
