Journal article
α-Fucosidases with different substrate specificities from two species of Fusarium
Department of Energy (DOE) Plant Research Lab and DOE Great Lakes Bioenergy Research Center, Michigan State University, East Lansing, MI 48824, USA.1
Two fungal-secreted α-fucosidases and their genes were characterized. FoFCO1 was purified from culture filtrates of Fusarium oxysporum strain 0685 grown on L-fucose and its encoding gene identified in the sequenced genome of strain 4287. FoFCO1 was active on p-nitrophenyl-α-fucoside (pNP-Fuc), but did not defucosylate a nonasaccharide (XXFG) fragment of pea xyloglucan.
A putative α-fucosidase gene (FgFCO1) from Fusarium graminearum was expressed in Pichia pastoris. FgFCO1 was ~1,800 times less active on pNP-Fuc than FoFCO1, but was able to defucosylate the XXFG nonasaccharide. Although FgFCO1 and FoFCO1 both belong to Glycosyl Hydrolase family 29, they share <25 % overall amino acid identity.
Alignment of all available fungal orthologs of FoFCO1 and FgFCO1 indicated that these two proteins belong to two subfamilies of fungal GH29 α-fucosidases. Fungal orthologs of subfamily 1 (to which FoFCO1 belongs) are taxonomically more widely distributed than subfamily 2 (FgFCO1), but neither was universally present in the sequenced fungal genomes.
Trichoderma reesei and most species of Aspergillus lack genes for either GH29 subfamily.
Language: | English |
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Publisher: | Springer-Verlag |
Year: | 2013 |
Pages: | 5371-5380 |
ISSN: | 14320614 and 01757598 |
Types: | Journal article |
DOI: | 10.1007/s00253-012-4423-3 |
4-nitrophenylfucoside Biotechnology Chemistry Chemistry and Materials Science DNA, Fungal Fucose Fusarium Fusarium graminearum Fusarium oxysporum Glycosides Microbial Genetics and Genomics Microbiology Molecular Sequence Data Sequence Alignment Sequence Analysis, DNA Sequence Homology, Amino Acid Substrate Specificity Xyloglucan alpha-L-Fucosidase