Journal article
Genome and chromosome identification in cultivated barley and related species of the Triticeae (Poaceae) by in situ hybridization with the GAA-satellite sequence
The satellite sequence studied was primarily composed of GAA repeats organized in long tracts of heterochromatic DNA. Fluorescent in situ hybridization (FISH) with the GAA satellite (GAA banding) to the chromosomes of barley, wheat, rye, and other Triticeae species produced banding patterns similar to those obtained by N-banding.
The GAA-banding patterns of barley are described in detail and those of 12 other Triticeae species are described briefly. In situ hybridization with the GAA-satellite sequence permits identification of all the chromosomes of barley. It is a valuable alternative to other banding techniques, especially in connection with physical gene mapping by FISH.
The application of the GAA-satellite sequence for the characterization of genomes in phylogenetic studies of genera containing the sequence is discussed.
| Language: | English |
|---|---|
| Year: | 1996 |
| Pages: | 93-104 |
| ISSN: | 14803321 and 08312796 |
| Types: | Journal article |
| DOI: | 10.1139/g96-013 |
| ORCIDs: | 0000-0002-9520-2232 and 0000-0001-6169-2504 |
Base Sequence Chromosome Banding Chromosome identification Cloning, Molecular DNA, Plant Edible Grain GAA-satellite sequence Genome differentiation Genome, Plant Hordeum Hordeum vulgare In Situ Hybridization, Fluorescence Karyotyping Molecular Sequence Data Sequence Analysis, DNA Trinucleotide Repeats Triticeae
