Journal article
Interlaboratory Trial for Measurement of Vitamin D and 25-Hydroxyvitamin D [25(OH)D] in Foods and a Dietary Supplement Using Liquid Chromatography–Mass Spectrometry
Nutrient Data Laboratory1
Biochemistry Department (0308)2
Chemical Sciences Division3
Food and Nutrition Laboratory4
Division of Food Chemistry, National Food Institute5
Suite 4400, 2711 South Loop Drive, Ames, Iowa 500106
3301 Kinsman Boulevard, Madison, Wisconsin 537047
Office of Dietary Supplements8
Food Composition and Method Development Laboratory9
Assessment of total vitamin D intake from foods and dietary supplements (DSs) may be incomplete if 25-hydroxyvitamin D [25(OH)D] intake is not included. However, 25(OH)D data for such intake assessments are lacking, no food or DS reference materials (RMs) are available, and comparison of laboratory performance has been needed.
The primary goal of this study was to evaluate whether vitamin D3 and 25(OH)D3 concentrations in food and DS materials could be measured with acceptable reproducibility. Five experienced laboratories from the United States and other countries participated, all using liquid chromatography tandem–mass spectrometry but no common analytical protocol; however, various methods were used for determining vitamin D3 in the DS.
Five animal-based materials (including three commercially available RMs) and one DS were analyzed. Reproducibility results for the materials were acceptable. Thus, it is possible to obtain consistent results among experienced laboratories for vitamin D3 and 25(OH)D3 in foods and a DS.
Language: | English |
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Publisher: | American Chemical Society |
Year: | 2016 |
Pages: | 3167-3175 |
ISSN: | 15205118 and 00218561 |
Types: | Journal article |
DOI: | 10.1021/acs.jafc.5b05016 |