Journal article
CLUSEAN: a computer-based framework for the automated analysis of bacterial secondary metabolite biosynthetic gene clusters
Eberhard-Karls-Universität Tübingen, Fakultät für Biologie, Mikrobiologie/Biotechnologie, Tübingen, Germany. tilmann.weber@biotech.uni-tuebingen.de1
Bacterial secondary metabolites are an important source of antimicrobial and cytostatic drugs. These molecules are often synthesized in a stepwise fashion by multimodular megaenzymes that are encoded in clusters of genes encoding enzymes for precursor supply and modification. In this work,we present an open source software pipeline, CLUSEAN (CLUster SEquence ANalyzer) that helps to annotate and analyze such gene clusters.
CLUSEAN integrates standard analysis tools, like BLAST and HMMer, with specific tools for the identification of the functional domains and motifs in nonribosomal peptide synthetases (NRPS)/type I polyketide synthases (PKS) and the prediction of specificities of NRPS.
Language: | English |
---|---|
Year: | 2009 |
Pages: | 13-17 |
ISSN: | 18734863 and 01681656 |
Types: | Journal article |
DOI: | 10.1016/j.jbiotec.2009.01.007 |
Annotation Anti-Bacterial Agents Antibiotics Bacteria Bacterial Proteins Computer Simulation Databases, Genetic Genes, Bacterial Macrolides Multigene Family NRPSpredictor Nonribosomal peptide synthesis Peptide Biosynthesis, Nucleic Acid-Independent Polyketide Polyketide Synthases Protein Structure, Tertiary Software