Journal article
Identification and Typing of Vibrio anguillarum: A Comparison of Different Methods
From the Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, Scotland1
Departament de Microbiologia, Universitat de Barcelona, Av. Diagonal 645, 08028 Barcelona, Spain2
Centre National de Typage Moleculaire Entérique, Unité des Entérobactéries, Institut Pasteur, 28 rue du Docteur Roux, F-75724 Paris Cedex 15, France3
Department of Veterinary Microbiology, Laboratory of Fish Diseases, Royal Veterinary and Agricultural University, Bülowsvej 13, DK-1870 Frederiksberg C, Denmark4
Laboratorium voor Micobiologie, Faculteit Wetenschappen, Universiteit Gent, K.L. Ledeganckstraar 35, B-9000 Gent, Belgium5
The majority (91%) of 260 isolates initially identified as Vibrio anguillarum, that were obtained from a wide range of hosts, habitats and geographical locations, were recovered in a single cluster based on the ribotype and were pathogenic to Atlantic salmon. A significant proportion of isolates (78% of the total) were allocated to 15 serogroups (O1–O10 and five previously undescribed groups referred to as VaNT1, VaNT2, VaNT4, NaNT5 and VaNT7).
A minority of isolates (6%) reacted with more than one antiserum or were self-agglutinating, and the remainder did not react with any of the antisera tested. Good correlation was noted between serogroups and lipopolysaccharide profiles, particularly with respect to isolates belonging to serogroups O1, 02 and 04ߝ010.
Plasmids were recognized in some serogroups. especially O1, which contained the 67 kb plasmid associated with virulence. However, the 19 profiles based on outer membrane protein patterns did not correspond to the results obtained with the other typing methods. Generally, the isolates were heterogeneous in their biochemical characteristics; 117 profiles were obtained with the API 20E system, and 9 and 32 clusters recognised from the results of BIOLOG fingerprinting and Biotype-100 biotyping methods, respectively.
Three dominant dusters were defined from fatty acid methyl esters profiles.
Language: | English |
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Year: | 1995 |
Pages: | 285-302 |
ISSN: | 16180984 and 07232020 |
Types: | Journal article |
DOI: | 10.1016/S0723-2020(11)80400-5 |