Journal article
Structure and chromosomal localization of the gene encoding barley seed peroxidase BP 2A
A clone, lambda Prx6.1, coding for a barley seed peroxidase (BP; EC 1.11.1.7), was isolated from a genomic library using a cDNA coding for the barley seed peroxidase, BP 1, as a probe. The nucleotide sequence coded for a BP showing 73% amino acid (aa) sequence identity with BP 1 and less than 50% similarity with other sequenced plant peroxidases.
The aa composition is 92% identical to that determined for BP 2 purified from mature barley grains, and therefore the gene product is named BP 2A. The alignment suggests that the coding region is interrupted by a 76-bp intron having the consensuses GT and AG, at the 5' and 3' ends, respectively. Alignment with BP 1 suggests that BP 2A has a leader peptide of 36 aa and the mature protein is 319 aa.
Alanine and leucine account for 50% of the residues of the leader peptide. Of the codons used 90% have a C or G in the third position. The promoter shows a putative abscisic acid-response element, 5'-GTACGTGTC, 115 bp upstream from the start codon. The BP 2A-encoding gene was RFLP-mapped on barley chromosome 3, and we suggest for this peroxidase locus the name Prx6.
Language: | English |
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Year: | 1992 |
Pages: | 261-266 |
ISSN: | 03781119 and 18790038 |
Types: | Journal article |
DOI: | 10.1016/0378-1119(92)90197-w |
ORCIDs: | 0000-0001-6169-2504 |
Amino Acid Sequence Base Sequence C-terminal processing Cloning, Molecular Genomic Library Hordeum Hordeum vulgare Molecular Sequence Data Peroxidases Plant Proteins Polymorphism, Restriction Fragment Length Promoter Regions, Genetic Protein Sorting Signals RFLP abscisic acid barley peroxidase intron nucleotide sequence signal peptide