Journal article
A catalog of the mouse gut metagenome
BGI Group1
National Institute for Agronomic Research2
King's College London3
University of Copenhagen4
Pfizer5
National Food Institute, Technical University of Denmark6
Chinese Academy of Sciences7
Chinese University of Hong Kong8
University of Gothenburg9
Department of Systems Biology, Technical University of Denmark10
Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark11
...and 1 moreWe established a catalog of the mouse gut metagenome comprising ∼2.6 million nonredundant genes by sequencing DNA from fecal samples of 184 mice. To secure high microbiome diversity, we used mouse strains of diverse genetic backgrounds, from different providers, kept in different housing laboratories and fed either a low-fat or high-fat diet.
Similar to the human gut microbiome, >99% of the cataloged genes are bacterial. We identified 541 metagenomic species and defined a core set of 26 metagenomic species found in 95% of the mice. The mouse gut microbiome is functionally similar to its human counterpart, with 95.2% of its Kyoto Encyclopedia of Genes and Genomes (KEGG) orthologous groups in common.
However, only 4.0% of the mouse gut microbial genes were shared (95% identity, 90% coverage) with those of the human gut microbiome. This catalog provides a useful reference for future studies.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group US |
| Year: | 2015 |
| Pages: | 1103-1108 |
| Journal subtitle: | Science and Business of Biotechnology |
| ISSN: | 15461696 and 10870156 |
| Types: | Journal article |
| DOI: | 10.1038/nbt.3353 |
| ORCIDs: | Licht, Tine Rask , Nielsen, H Bjørn , 0000-0002-3592-126X , 0000-0002-0886-9101 , 0000-0003-3194-3805 , 0000-0003-2777-6725 and 0000-0002-6024-0917 |
