Journal article
In vitro toxicity of cationic micelles and liposomes in cultured human hepatocyte (HepG2) and lung epithelial (A549) cell lines
University of Copenhagen1
H. Lundbeck A/S2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Colloids and Biological Interfaces, Department of Micro- and Nanotechnology, Technical University of Denmark4
National Food Institute, Technical University of Denmark5
Office for Innovation & Sector Services, Administration, Technical University of Denmark6
The aim of this study was to compare the effects of cationic micelle and liposome drug delivery systems on liver and lung cells in a toxicological in vitro screening model, with observations on cytotoxicity and genotoxicity. A screening battery was established for assessment of a broad range of parameters related to adverse effects.
Clear concentration response effects were observed related to impairment of mitochondrial function, membrane integrity and oxidative stress markers, but no effect was observed on genotoxicity. The adverse effects were highest for the liposomes. The High Content Screening seems optimal for initial screening of adverse effects, and combined with standard cytotoxicity measurements initial screening can be performed for predictive toxicological screening.
| Language: | English |
|---|---|
| Year: | 2016 |
| Pages: | 164-171 |
| ISSN: | 18793177 and 08872333 |
| Types: | Journal article |
| DOI: | 10.1016/j.tiv.2016.08.002 |
| ORCIDs: | 0000-0002-7206-0032 , 0000-0002-6514-8407 , 0000-0001-9552-8518 , 0000-0002-2021-1249 and Andresen, Thomas Lars |
Comet assay Drug delivery systems Hepatocytes In vitro toxicology Journal Article Liposomes Lung epithelial cells Micelles Nanomedicine
1,2-dioleoyloxy-3-(trimethylammonium)propane A549 Cells Cell Survival Cholesterol Drug Delivery Systems Epithelial Cells Fatty Acids, Monounsaturated Hep G2 Cells Humans IL6 protein, human Interleukin-6 Lung Polyethylene Glycols Polymethacrylic Acids Quaternary Ammonium Compounds Tumor Necrosis Factor-alpha
