Journal article
β-Hydroxybutyrate dehydrogenase decorated MXene nanosheets for the amperometric determination of β-hydroxybutyrate
Chung-Ang University1
Department of Health Technology, Technical University of Denmark2
Experimental & Translational Immunology, Department of Health Technology, Technical University of Denmark3
Nanomaterials and Nanobiosensors, Experimental & Translational Immunology, Department of Health Technology, Technical University of Denmark4
MXene nanosheets of type Ti3C2Tx were modified with β-hydroxybutyrate dehydrogenase and then used as a biosensor for amperometric sensing of β-hydroxybutyrate. The MXene and the nanocomposite were characterized by X-ray photoelectron spectroscopy, field-emission scanning electron microscopy, X-ray diffraction, and Fourier transform infrared spectroscopy.
The MXene has a layered structure and proved to be an excellent immobilization matrix providing good compatibility with the enzyme β-hydroxybutyrate dehydrogenase. The MXene-based biosensor, best operated at a potential of − 0.35 V (vs. Ag/AgCl), displays a wide linear range (0.36 to 17.9 mM), a sensitivity of 0.480 μA mM−1 cm−2, and a low detection limit (45 μM).
The biosensor was successfully applied to the determination of β-hydroxybutyrate in (spiked) real serum samples. [Figure not available: see fulltext.].
| Language: | English |
|---|---|
| Publisher: | Springer Vienna |
| Year: | 2020 |
| Pages: | 277 |
| Journal subtitle: | Analytical Sciences Based on Micro- and Nanomaterials |
| ISSN: | 00768642 , 00263672 and 14365073 |
| Types: | Journal article |
| DOI: | 10.1007/s00604-020-04258-y |
| ORCIDs: | Mohammadniaei, Mohsen , 0000-0001-7872-8489 , 0000-0002-3052-5524 , 0000-0003-0054-6188 , 0000-0003-0808-8628 and 0000-0002-7028-4745 |
