Journal article
Simultaneous quantification of vitamin D3, 25-hydroxyvitamin D-3 and 24,25-dihydroxyvitamin D3 in human serum by LC-MS/MS
Introduction. Serum 25-hydroxy-vitamin D is the established biomarker of vitamin D status although serum concentrations of vitamin D and 24,25-dihydroxyvitamin D may also be of interest to understand the in vivo kinetics of serum 25-hydroxyvitamin D. Method. An LC-MS/MS method was developed and validated to quantify vitamin D-3, 25-hydroxyvitamin D-3 and 24,25-dihydroxyvitamin D-3 in serum.
After protein precipitation of the serum it was loaded on a HybridSPE column to separate vitamin D metabolites from phospholipids. Vitamin D-3, 25-hydroxyvitamin D-3 and 24,25-dihydroxyvitamin D-3 in the eluate were derivatized by 4-phenyl-1,2,4-triazoline-3,5-dione to improve sensitivity in the following LC-MS/MS analysis.
Results. Using only 100 L serum the limit of quantification was
| Language: | English |
|---|---|
| Publisher: | Taylor & Francis |
| Year: | 2014 |
| Pages: | 418-423 |
| ISSN: | 15027686 , 00365513 , 21661030 and 0085591x |
| Types: | Journal article |
| DOI: | 10.3109/00365513.2014.900694 |
| ORCIDs: | Frandsen, Henrik Lauritz and Jakobsen, Jette |
