Journal article
Competitive protein adsorption to polymer surface from human serum
Polymer Microsystems for Electrophysiology Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Risø National Laboratory for Sustainable Energy, Technical University of Denmark3
Solar Energy Programme, Risø National Laboratory for Sustainable Energy, Technical University of Denmark4
Polymer Microsystems for Cell Processing Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
Radioecology and Tracer Studies, Radiation Research Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark6
Radiation Research Division, Risø National Laboratory for Sustainable Energy, Technical University of Denmark7
Surface modification by "soft" plasma polymerisation to obtain a hydrophilic and non-fouling polymer surface has been validated using radioactive labelling. Adsorption to unmodified and modified polymer surfaces, from both single protein and human serum solutions, has been investigated. By using different radioisotopes, albumin and Immunoglobulin G (IgG) adsorption has been monitored simultaneously during competitive adsorption processes, which to our knowledge has not been reported in the literature before.
Results show that albumin and IgG adsorption is dependent on adsorption time and on the presence and concentration of other proteins in bulk solutions during adsorption. Generally, lower albumin and IgG adsorption was observed on the modified and more hydrophilic polymer surfaces, but otherwise the modified and unmodified polymer surfaces showed the same adsorption characteristics.
| Language: | English |
|---|---|
| Publisher: | Springer US |
| Year: | 2008 |
| Pages: | 2179-2185 |
| Journal subtitle: | Official Journal of the European Society for Biomaterials |
| ISSN: | 15734838 and 09574530 |
| Types: | Journal article |
| DOI: | 10.1007/s10856-007-3318-9 |
| ORCIDs: | Larsen, Niels Bent and Hou, Xiaolin |
Adsorption Albumins Biocompatible Materials Biomaterials Biophysics Ceramics, Glass, Composites, Natural Methods Characterization and Evaluation of Materials Chemistry Ethers Ethylene Glycols Former Faculty of Pharmaceutical Sciences Humans Immunoglobulin G Metallic Materials Polymer Sciences Polymers Protein Binding Protein Conformation Proteins Serum Surface Properties Surfaces and Interfaces, Thin Films Time Factors Vinyl Compounds diethylene glycol diethylene glycol vinyl ether
