Conference paper
The growth rate of pyrimidine auxotrophic mutants of Lactococcus lactis MG1363 is reduced in the presence of exogenous aspartate
Nucleotide metabolism is important for all cells as supplier of building blocks for the synthesis of nucleic acids and coenzymes. Furthermore, they act as intracellular energy carriers and allosteric effectors in a large number of enzymatic reactions. Nucleotides can either be made de novo or from preformed metabolites present in the growth medium.
In addition to the obvious phenotype of a mutant in the pyrimidine biosynthetic pathway - requirement for a pyrimidine precursor in the growth medium - pleiotrophic effects are often seen. The work presented here shows, that a mutation in one of the genes encoding enzymes in the distal part of the pyrimidine biosynthetic pathway of L. lactis MG1363, results in reduction of the growth rate if exogenous aspartate is supplied to the growth medium.
This observation can be explained by an increased accumulation of a toxic intermediate, most likely carbamoyl aspartate, provoked by high concentrations of aspartate.
Language: | English |
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Publisher: | American Society for Microbiology |
Year: | 1998 |
Pages: | 68-68 |
Proceedings: | 5th ASM Conference on Streptococcal Genetics |
Types: | Conference paper |
ORCIDs: | Martinussen, Jan |