Conference paper
MicroRNA Expression during Viral Infection or PolyI:C Stimulation in a Fish Model
National Veterinary Institute, Technical University of Denmark1
Section of Fish Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark2
Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark3
Aarhus University4
Fish are important as small vertebrate models for studying various aspects of development and disease. MicroRNA regulation in fish has so far received attention especially in studies of their expression and function during embryonic development. In the studies carried out at the National Veterinary Institute in Århus we aim at using fish models for studying microRNA regulation during viral infection.
In the studies presented here we make use of a qPCR method to detect miRNAs in fish cells. We present results regarding the expression of the immunologically relevant microRNAs, miR-155, miR-146a and miR-146b in fish cells during infection with the fish pathogenic virus viral hemorrhagic septicemia virus (VHSV) and during immune stimulation with double stranded RNA (polyI:C).
We highlight the need of finding stable normalization genes for microRNA detection.
| Language: | English |
|---|---|
| Year: | 2009 |
| Proceedings: | MicroRNA : MicroRNA research results, technology insights and networking |
| Types: | Conference paper |
| ORCIDs: | Lorenzen, Niels |
