Journal article
Multiple Soluble Components of the Glycocalyx Are Increased in Patient Plasma After Ischemic Stroke
Copenhagen University Hospital Herlev and Gentofte1
University of Copenhagen2
Department of Health Technology, Technical University of Denmark3
Biotherapeutic Engineering and Drug Targeting, Department of Health Technology, Technical University of Denmark4
Colloids & Biological Interfaces, Biotherapeutic Engineering and Drug Targeting, Department of Health Technology, Technical University of Denmark5
Background and Purpose- The GLX (glycocalyx) is a protein/polysaccharide meshwork at the cellular surface. Consisting largely of glycosaminoglycans and proteoglycans, the GLX can shed in response to stress. In this study, we assay 11 components of the GLX in plasma from patients with ischemic stroke from a longitudinal cohort.
Methods- Plasma samples from healthy individuals (N=8), and patients with ischemic stroke day ≥3, day 7, and day 90 (N=9-14) were immunoassayed for diverse components of the GLX. Results- Median stroke severity was mild (National Institutes of Health Stroke Scale 2.0 (range, 0-6) at day ≤3). Three (keratan-chondroitin-heparan-sulfate) of 4 glycosaminoglycans and CD44 (proteoglycan) were increased at day 7 and returned to baseline at day 90.
Proteoglycan syndecan (Syn)-3 increased and Syn-2 levels decreased, significantly. Conclusions- Individual GLX components are often assayed as stand-alone biomarkers for endothelial health. This study suggests a full assessment of GLX components is more indicative of the endothelial health of an individual and represents a complex GLX signature that may be valuable as a composite biomarker of disease.
Language: | English |
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Year: | 2019 |
Pages: | 2948-2951 |
ISSN: | 15244628 and 00392499 |
Types: | Journal article |
DOI: | 10.1161/STROKEAHA.119.025953 |
ORCIDs: | Hempel, Casper |