Journal article
Basics of antibody phage display technology
Tropical Pharmacology and Biotherapeutics, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark1
Department of Biotechnology and Biomedicine, Technical University of Denmark2
Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark3
Metabolic Signaling and Regulation, Section for Microbial and Chemical Ecology, Department of Biotechnology and Biomedicine, Technical University of Denmark4
Iontas Ltd.5
Network Engineering of Eukaryotic Cell factories, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark6
Antibody discovery has become increasingly important in almost all areas of modern medicine. Different antibody discovery approaches exist, but one that has gained increasing interest in the field of toxinology and antivenom research is phage display technology. In this review, the lifecycle of the M13 phage and the basics of phage display technology are presented together with important factors influencing the success rates of phage display experiments.
Moreover, the pros and cons of different antigen display methods and the use of naïve versus immunized phage display antibody libraries is discussed, and selected examples from the field of antivenom research are highlighted. This review thus provides in-depth knowledge on the principles and use of phage display technology with a special focus on discovery of antibodies that target animal toxins.
Language: | English |
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Publisher: | MDPI |
Year: | 2018 |
Pages: | 236 |
ISSN: | 20726651 |
Types: | Journal article |
DOI: | 10.3390/toxins10060236 |
ORCIDs: | Kilstrup, Mogens , Laustsen, Andreas Hougaard and Ledsgaard, Line |