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Journal article

The impact of structural integrity and route of administration on the antibody specificity against three cow's milk allergens - a study in Brown Norway rats

By Madsen, Jeanette Lund1; Kroghsbo, Stine2,3; Madsen, Charlotte Bernhard2,3; Pozdnyakova, Irina4; Barkholt, Vibeke5,6; Bøgh, Katrine Lindholm2,3

From

Technical University of Denmark1

National Food Institute, Technical University of Denmark2

Division of Toxicology and Risk Assessment, National Food Institute, Technical University of Denmark3

University of Copenhagen4

Department of Systems Biology, Technical University of Denmark5

Enzyme and Protein Chemistry, Department of Systems Biology, Technical University of Denmark6

This study showed that the three-dimensional (3D) structure has a significant impact on the antibodies raised for both systemic and orally administered allergens. A remarkable difference in the antibody binding patterns against linear and conformational epitope was seen between the allergens, indicating that the structural characteristics of proteins may heavily affect the induced antibody response.

Language: English
Publisher: BioMed Central
Year: 2014
Pages: 25
ISSN: 20457022
Types: Journal article
DOI: 10.1186/2045-7022-4-25
ORCIDs: Madsen, Charlotte Bernhard and Bøgh, Katrine Lindholm
Keywords

Animal model Conformational epitopes Cow’s milk allergens Food allergy IgE Linear epitopes Oral administration i.p. immunisation

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