Journal article
Compressed collagen constructs with optimized mechanical properties and cell interactions for tissue engineering applications
National Food Institute, Technical University of Denmark1
Research group for Nano-Bio Science, National Food Institute, Technical University of Denmark2
Department of Mechanical Engineering, Technical University of Denmark3
Isfahan University of Technology4
Karolinska Institutet5
Materials and Surface Engineering, Department of Mechanical Engineering, Technical University of Denmark6
In this study, we are introducing a simple, fast and reliable add-in to the technique of plastic compression (PC) to obtain collagen sheets with decreased fibrillar densities, representing improved cell-interactions and mechanical properties. Collagen hydrogels with different initial concentrations (1.64mg/mL-0.41mg/mL) were compressed around an electrospun sheet of PLGA.
The scaffolds were then studied as non-seeded, or seeded with 3T3 fibroblast cells and cultured for 7 days. Confocal microscopy and TEM imaging of non-seeded scaffolds showed that by decreasing the share of collagen in the hydrogel formula, collagen sheets with similar thickness but lower fibrous densities were achieved.
Nanomechanical characterization of compressed collagen sheets by AFM showed that Young's modulus was inversely proportional to the final concentration of collagen. Similarly, according to SEM, MTS, and cell nuclei counting, all the scaffolds supported cell adhesion and proliferation, whilst the highest metabolic activities and proliferation were seen in the scaffolds with lowest collagen content in hydrogel formula.
We conclude that by decreasing the collagen content in the formula of collagen hydrogel for plastic compression, not only a better cell environment and optimum mechanical properties are achieved, but also the application costs of this biopolymer is reduced.
Language: | English |
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Year: | 2018 |
Pages: | 158-166 |
ISSN: | 18790003 and 01418130 |
Types: | Journal article |
DOI: | 10.1016/j.ijbiomac.2017.11.117 |
ORCIDs: | Ajalloueian, Fatemeh , Lee, Seunghwan and Chronakis, Ioannis S. |
Collagen hydrogel Fibrillar density Fibroblast Infiltration Nanomechanical characteristics Plastic compression Proliferation
3T3 Cells Animals Biocompatible Materials Biomechanical Phenomena Cell Adhesion Cell Communication Cell Proliferation Collagen Hydrogels Lactic Acid Mechanical Phenomena Mice Polyglycolic Acid Polylactic Acid-Polyglycolic Acid Copolymer Tensile Strength Tissue Engineering Tissue Scaffolds