Journal article
Verification of cell viability in bioengineered tissues and organs before clinical transplantation
Advanced Center for Translational Regenerative Medicine (ACTREM), Karolinska Institutet, Huddinge, Stockholm, Sweden.1
Department of Molecular Medicine and Surgery, Division of Cardiothoracic Surgery, Karolinska Institutet, Karolinska University Hospital, Solna, Stockholm, Sweden.2
University of Rome "Tor Vergata", Department of Industrial Engineering, Intrauniversitary Consortium for Material Science and Technology (INSTM), Research Unit "Tor Vergata", Rome, Italy.3
Center for Bioscience, Department of Biosciences and Nutrition, Karolinska Institutet, Huddinge, Stockholm, Sweden.4
Advanced Center for Translational Regenerative Medicine (ACTREM), Karolinska Institutet, Huddinge, Stockholm, Sweden. Electronic address: paolo.macchiarini@ki.se.5
The clinical outcome of transplantations of bioartificial tissues and organs depends on the presence of living cells. There are still no standard operative protocols that are simple, fast and reliable for confirming the presence of viable cells on bioartificial scaffolds prior to transplantation. By using mathematical modeling, we have developed a colorimetric-based system (colorimetric scale bar) to predict the cell viability and density for sufficient surface coverage.
First, we refined a method which can provide information about cell viability and numbers in an in vitro setting: i) immunohistological staining by Phalloidin/DAPI and ii) a modified colorimetric cell viability assay. These laboratory-based methods and the developed colorimetric-based system were then validated in rat transplantation studies of unseeded and seeded tracheal grafts.
This was done to provide critical information on whether the graft would be suitable for transplantation or if additional cell seeding was necessary. The potential clinical impact of the colorimetric scale bar was confirmed using patient samples. In conclusion, we have developed a robust, fast and reproducible colorimetric tool that can verify and warrant viability and integrity of an engineered tissue/organ prior to transplantation.
This should facilitate a successful transplantation outcome and ensure patient safety.
Language: | English |
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Year: | 2013 |
Pages: | 4057-4067 |
ISSN: | 18785905 and 01429612 |
Types: | Journal article |
DOI: | 10.1016/j.biomaterials.2013.02.057 |
Animals Bioartificial Organs Bioengineering Cell Adhesion Cell Count Cell Survival Female Humans Male Mesenchymal Stem Cells Microscopy, Electron, Scanning Polyurethanes Rats Rats, Sprague-Dawley Reproducibility of Results Tissue Scaffolds Trachea Translational Research, Biomedical Transplantation Young Adult