Journal article
Common variants in CYP2R1 and GC genes are both determinants of serum 25-hydroxyvitamin D concentrations after UVB irradiation and after consumption of vitamin D3-fortified bread and milk during winter in Denmark
National Food Institute, Technical University of Denmark1
Division of Nutrition, National Food Institute, Technical University of Denmark2
National Research Centre for the Working Environment3
Division of Toxicology and Risk Assessment, National Food Institute, Technical University of Denmark4
Department of Applied Mathematics and Computer Science, Technical University of Denmark5
Statistics and Data Analysis, Department of Applied Mathematics and Computer Science, Technical University of Denmark6
Aarhus University7
Holbæk Hospital8
Copenhagen University Hospital Herlev and Gentofte9
Background: Little is known about how the genetic variation in vitamin D modulating genes influences ultraviolet (UV)B–induced 25-hydroxyvitamin D [25(OH)D] concentrations. In the Food with vitamin D (VitmaD) study, we showed that common genetic variants rs10741657 and rs10766197 in 25-hydroxylase (CYP2R1) and rs842999 and rs4588 in vitamin D binding protein (GC) predict 25(OH)D concentrations at late summer and after 6-mo consumption of cholecalciferol (vitamin D3)–fortified bread and milk.
Objectives: In the current study, called the Vitamin D in genes (VitDgen) study, we analyzed associations between the increase in 25(OH)D concentrations after a given dose of artificial UVB irradiation and 25 single nucleotide polymorphisms located in or near genes involved in vitamin D synthesis, transport, activation, or degradation as previously described for the VitmaD study.
Second, we aimed to determine whether the genetic variations in CYP2R1 and GC have similar effects on 25(OH)D concentrations after artificial UVB irradiation and supplementation by vitamin D3–fortified bread and milk. Design: The VitDgen study includes 92 healthy Danes who received 4 whole-body UVB treatments with a total dose of 6 or 7.5 standard erythema doses during a 10-d period in winter.
The VitmaD study included 201 healthy Danish families who were given vitamin D3–fortified bread and milk or placebo for 6 mo during the winter. Results: After UVB treatments, rs10741657 in CYP2R1 and rs4588 in GC predicted UVB-induced 25(OH)D concentrations as previously shown in the VitmaD study. Compared with noncarriers, carriers of 4 risk alleles of rs10741657 and rs4588 had lowest concentrations and smallest increases in 25(OH)D concentrations after 4 UVB treatments and largest decreases in 25(OH)D concentrations after 6-mo consumption of vitamin D3–fortified bread and milk.
Conclusion: Common genetic variants in the CYP2R1 and GC genes modify 25(OH)D concentrations in the same manner after artificial UVB-induced vitamin D and consumption of vitamin D3–fortified bread and milk. The VitDgen study was registered at clinicaltrials.gov as NCT01741233. The VitmaD study was registered at clinicaltrials.gov as NCT01184716.
| Language: | English |
|---|---|
| Publisher: | American Society for Clinical Nutrition |
| Year: | 2014 |
| Pages: | 218-227 |
| ISSN: | 19383207 and 00029165 |
| Types: | Journal article |
| DOI: | 10.3945/ajcn.114.092148 |
| ORCIDs: | Nissen, Ioanna , Ravn-Haren, Gitte , Andersen, Rikke and Mejborn, Heddie |
25-Hydroxyvitamin D 2 25-hydroxyvitamin D Adolescent Adult Animals Bread CYP2R1 protein, human Calcifediol Child Cholecalciferol Cholestanetriol 26-Monooxygenase Cytochrome P450 Family 2 Denmark Double-Blind Method Food, Fortified Genetic Association Studies Humans Male Middle Aged Milk Polymorphism, Single Nucleotide SNPs Seasons Skin UVB radiation Ultraviolet Therapy Vitamin D Deficiency Vitamin D-Binding Protein Whole-Body Irradiation Young Adult genetic polymorphism vitamin D status vitamin D supplements
