Journal article
Complex I is bypassed during high intensity exercise
Chalmers University of Technology1
University of Gothenburg2
Swedish School of Sport and Health Sciences3
Yeast Cell Factories, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
Human muscles are tailored towards ATP synthesis. When exercising at high work rates muscles convert glucose to lactate, which is less nutrient efficient than respiration. There is hence a trade-off between endurance and power. Metabolic models have been developed to study how limited catalytic capacity of enzymes affects ATP synthesis.
Here we integrate an enzyme-constrained metabolic model with proteomics data from muscle fibers. We find that ATP synthesis is constrained by several enzymes. A metabolic bypass of mitochondrial complex I is found to increase the ATP synthesis rate per gram of protein compared to full respiration. To test if this metabolic mode occurs in vivo, we conduct a high resolved incremental exercise tests for five subjects.
Their gas exchange at different work rates is accurately reproduced by a whole-body metabolic model incorporating complex I bypass. The study therefore shows how proteome allocation influences metabolism during high intensity exercise.
Language: | English |
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Publisher: | Nature Publishing Group UK |
Year: | 2019 |
Pages: | 5072 |
ISSN: | 20411723 |
Types: | Journal article |
DOI: | 10.1038/s41467-019-12934-8 |
ORCIDs: | 0000-0002-9476-4516 , 0000-0002-0003-6463 , 0000-0002-7743-9295 , 0000-0002-1343-8656 and 0000-0002-9955-6003 |