About

Log in?

DTU users get better search results including licensed content and discounts on order fees.

Anyone can log in and get personalized features such as favorites, tags and feeds.

Log in as DTU user Log in as non-DTU user No thanks

DTU Findit

Journal article

3D H2BC: A novel experiment for small-molecule and biomolecular NMR at natural isotopic abundance

From

Carlsberg Research Center1

Novo Nordisk Foundation2

Department of Chemistry, Technical University of Denmark3

3D H2BC is introduced for heteronuclear assignment on natural abundance samples even for biomolecules up to at least 10 kDa in low millimolar concentrations as an overnight experiment using the latest generation of cryogenically cooled probes. The short pulse sequence duration of H2BC is maintained in the 3D version due to multiple use of the constant-time delay.

Applications ranging from a small lipid to a non-recombinant protein demonstrate the merits of 3D H2BC and the ease of obtaining assignments in chains of protonated carbons.

Language: English
Year: 2009
Pages: 340-343
ISSN: 10960856 and 10907807
Types: Journal article
DOI: 10.1016/j.jmr.2009.06.017
ORCIDs: Duus, Jens Øllgaard

DTU users get better search results including licensed content and discounts on order fees.

Log in as DTU user

Access

Analysis