Journal article
Parameters necessary to define an immobilized enzyme preparation
University of Lorraine1
Department of Chemical and Biochemical Engineering, Technical University of Denmark2
PROSYS - Process and Systems Engineering Centre, Department of Chemical and Biochemical Engineering, Technical University of Denmark3
KT Consortium, Department of Chemical and Biochemical Engineering, Technical University of Denmark4
CSIC5
Biocatalytic processes continue to find increasing application in industry. Therefore enzyme immobilization has also become of increasing importance as a means of allowing enzyme containment within reactors operating in continuous mode or else separation of enzyme after use in (fed-)batch reactors, as well as potential recycle.
Whilst much has been reported in the scientific literature about enzyme immobilization methods, in many cases the protocol leads to losses in enzyme activity. In this review we outline the reasons for loss of activity during immobilization and highlight suitable diagnostic tests to elucidate the precise cause and thereby methods to restore activity.
The need for standardized reporting of immobilization methods is also emphasized as a means of benchmarking alternative approaches.
Language: | English |
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Year: | 2020 |
Pages: | 66-80 |
ISSN: | 18733298 and 13595113 |
Types: | Journal article |
DOI: | 10.1016/j.procbio.2019.11.026 |
ORCIDs: | Woodley, John M. |