Journal article
Identification of a novel, invasive, not-yet-cultivated Treponema sp in the large intestine of pigs by PCR amplification of the 16S rRNA gene
Microbial Ecology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark1
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Section for Veterinary Diagnostics, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4
Laser capture microdissection in combination with fluorescent in situ hybridization was used to identify an unknown species of spirochetes from the pig colonic mucosa. The 16S rRNA gene was PCR amplified, and the closest related type strain was Treponema bryantii(T) (90.1%). The spirochete, here named "Candidatus Treponema suis," was associated with colitis, including invasion of the surface epithelium as well as superficial parts of the mucosa.
| Language: | English |
|---|---|
| Publisher: | American Society for Microbiology |
| Year: | 2006 |
| Pages: | 4537-4540 |
| ISSN: | 1098660x , 00951137 and 1070633x |
| Types: | Journal article |
| DOI: | 10.1128/JCM.01537-06 |
| ORCIDs: | Jensen, Tim Kåre and Boye, Mette |
Animals Colitis Colon DNA, Bacterial DNA, Ribosomal Genes, rRNA In Situ Hybridization, Fluorescence Intestinal Mucosa Microdissection Molecular Sequence Data Phylogeny Polymerase Chain Reaction RNA, Ribosomal, 16S Sequence Analysis, DNA Sequence Homology, Nucleic Acid Swine Swine Diseases Treponema Treponemal Infections
