Journal article
Measurement of bacterial gene expression in vivo by laser capture microdissection and quantitative real-time RT-PCR
Microbial Ecology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark1
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2
National Veterinary Institute, Technical University of Denmark3
Bacteriology & Pathology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark4
Due to the relative small number of bacterial pathogens present in an infected host, exploration of pathogen gene expression in vivo is challenging. This study reports the development of a protocol for quantifying bacterial gene expression in vivo in Actinobacillus pleuropneumoniae using laser capture microdissection and real-time quantitative RT-PCR.
| Language: | English |
|---|---|
| Year: | 2007 |
| Pages: | 414-416 |
| ISSN: | 18728359 and 01677012 |
| Types: | Journal article |
| DOI: | 10.1016/j.mimet.2006.12.003 |
| ORCIDs: | Jensen, Tim Kåre and Boye, Mette |
