Journal article
Extraction, enrichment, and in situ electrochemical detection on lab- on-a-disc; monitoring the production of a bacterial secondary metabolite
Department of Biotechnology and Biomedicine, Technical University of Denmark1
Department of Health Technology, Technical University of Denmark2
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
Bacterial Cell Factory Optimization, Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
Research Groups, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
Nanoprobes, Drug Delivery and Sensing, Department of Health Technology, Technical University of Denmark6
Drug Delivery and Sensing, Department of Health Technology, Technical University of Denmark7
Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark8
Development of microsystems, which enable 'sample-to-answer' detection from real samples, is often challenging. We present the first integration of supported liquid membrane extraction combined with electrochemical detection on a centrifugal fluidic platform. The developed lab-on-a-disc (LoD) system enabled the separation, enrichment and subse-quent electrochemical detection of the target analyte from a complex sample mixture.
As a case study, we quantified the amount of a dietary supplement and pharmaceutical precursor, p-Coumaric acid, from bacterial growth media at different time points during production. The assay, extraction, and detection, performed on the LoD device, proved to be a low cost and environmentally friendly approach, requiring only a few tens of µL organic solvent and enabled detection in 3 µL volume.
In addition, the data obtained from the centrifugal platform showed a good correlation with data obtained from high performance liquid chromatography analysis.
Language: | English |
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Publisher: | American Chemical Society |
Year: | 2019 |
Pages: | 398-405 |
ISSN: | 23793694 |
Types: | Journal article |
DOI: | 10.1021/acssensors.8b01277 |
ORCIDs: | Jendresen, Christian Bille , Nielsen, Alex Toftgaard , Emnéus, Jenny , Boisen, Anja , Zor, Kinga and 0000-0003-0094-4063 |