Conference paper
Investigation of cleaning and regeneration methods for reliable construction of DNA cantilever biosensors
Department of Micro- and Nanotechnology, Technical University of Denmark1
Nanoprobes, Department of Micro- and Nanotechnology, Technical University of Denmark2
BioLabChip, Department of Micro- and Nanotechnology, Technical University of Denmark3
Bioanalytics, Department of Micro- and Nanotechnology, Technical University of Denmark4
McGill University5
Biosensing systems based on detecting changes in cantilever surface stress have attracted great interest. To achieve high reliability of measurements, high quality and high reproducibility in functionalization of the sensor surface are key points. In this paper, we investigate different methods to clean and regenerate the sensing surface of cantilever biosensors.
Perchloric acid potential sweep, potassium hydroxide-hydrogen peroxide, and piranha cleaning are investigated here. Peak-current potential differences from cyclic voltammetry, X-ray photo-electron spectroscopy and fluorescence detection are applied to characterize surface cleanliness. The experimental results show that piranha cleaning is the most reliable and efficient cleaning procedure.
Language: | English |
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Year: | 2012 |
Proceedings: | 9th International Nanomechanical Sensing Workshop |
Types: | Conference paper |
ORCIDs: | Yi, Sun , Heiskanen, Arto , Wolff, Anders and Boisen, Anja |