Journal article
Measuring glucose cerebral metabolism in the healthy mouse using hyperpolarized C-13 magnetic resonance
Swiss Federal Institute of Technology Lausanne1
University of Texas Southwestern Medical Center2
Department of Electrical Engineering, Technical University of Denmark3
Center for Magnetic Resonance, Department of Electrical Engineering, Technical University of Denmark4
Center for Hyperpolarization in Magnetic Resonance, Centers, Technical University of Denmark5
The mammalian brain relies primarily on glucose as a fuel to meet its high metabolic demand. Among the various techniques used to study cerebral metabolism, C-13 magnetic resonance spectroscopy (MRS) allows following the fate of C-13-enriched substrates through metabolic pathways. We herein demonstrate that it is possible to measure cerebral glucose metabolism in vivo with sub-second time resolution using hyperpolarized C-13 MRS.
In particular, the dynamic C-13-labeling of pyruvate and lactate formed from C-13-glucose was observed in real time. An ad-hoc synthesis to produce [2,3,4,6,6-H-2(5), 3,4-C-13(2)]-D-glucose was developed to improve the 13C signal-to-noise ratio as compared to experiments performed following [U-H-2(7), U-C-13]-D-glucose injections.
The main advantage of only labeling C3 and C4 positions is the absence of C-13-C-13 coupling in all downstream metabolic products after glucose is split into 3-carbon intermediates by aldolase. This unique method allows direct detection of glycolysis in vivo in the healthy brain in a noninvasive manner.
Language: | English |
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Publisher: | Nature Publishing Group UK |
Year: | 2017 |
Pages: | 11719 |
ISSN: | 20452322 |
Types: | Journal article |
DOI: | 10.1038/s41598-017-12086-z |
ORCIDs: | 0000-0002-8484-3448 , Karlsson, Magnus and Lerche, Mathilde Hauge |