Journal article
Rapid detection and identification of Stachybotrys and Chaetomium species using tissue PCR analysis
Department of Systems Biology, Technical University of Denmark1
Aalborg University2
Department of Civil Engineering, Technical University of Denmark3
Section for Indoor Climate and Building Physics, Department of Civil Engineering, Technical University of Denmark4
Eucaryotic Molecular Cell Biology, Department of Systems Biology, Technical University of Denmark5
Indoor fungi are a worldwide problem causing negative health effects for infected building's occupants and even deterioration of building structures. Different fungal species affect buildings and their inhabitants differently. Therefore, rapid and accurate identification of fungi to the species level is essential for health risk assessment and building remediation.
This study focuses on molecular identification of two common indoor fungal genera: Stachybotrys and Chaetomium. This study proposes two new DNA barcode candidates for Stachybotrys and Chaetomium: the gene encoding mitogen activated protein kinase (hogA) and the intergenic region between histone 3 and histone 4 (h3-h4) as well as it introduces a rapid - 3.5 h - protocol for direct Stachybotrys and Chaetomium species identification, which bypasses culture cultivation, DNA extraction and DNA sequencing.
Language: | English |
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Year: | 2016 |
Pages: | 115-122 |
ISSN: | 18728359 and 01677012 |
Types: | Journal article |
DOI: | 10.1016/j.mimet.2016.09.005 |
ORCIDs: | 0000-0002-6514-4828 , Rode, Carsten , Andersen, Birgitte and Hoof, Jakob Blæsbjerg |