Journal article
Convergent evolution and adaptation of Pseudomonas aeruginosa within patients with cystic fibrosis
Department of Systems Biology, Technical University of Denmark1
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark2
Bacterial Cell Factories, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark3
Infection Microbiology, Department of Systems Biology, Technical University of Denmark4
Little is known about how within-host evolution compares between genotypically different strains of the same pathogenic species. We sequenced the whole genomes of 474 longitudinally collected clinical isolates of Pseudomonas aeruginosa sampled from 34 children and young individuals with cystic fibrosis.
Our analysis of 36 P. aeruginosa lineages identified convergent molecular evolution in 52 genes. This list of genes suggests a role in host adaptation for remodeling of regulatory networks and central metabolism, acquisition of antibiotic resistance and loss of extracellular virulence factors. Furthermore, we find an ordered succession of mutations in key regulatory networks.
Accordingly, mutations in downstream transcriptional regulators were contingent upon mutations in upstream regulators, suggesting that remodeling of regulatory networks might be important in adaptation. The characterization of genes involved in host adaptation may help in predicting bacterial evolution in patients with cystic fibrosis and in the design of future intervention strategies.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group US |
| Year: | 2015 |
| Pages: | 57-64 |
| ISSN: | 15461718 and 10614036 |
| Types: | Journal article |
| DOI: | 10.1038/ng.3148 |
| ORCIDs: | Marvig, Rasmus Lykke , Madsen Sommer, Lea Mette and Molin, Søren |
Adaptation, Biological Adolescent Adult Bacterial Proteins Carrier State Child Child, Preschool Clone Cells Cystic Fibrosis Evolution, Molecular Female Follow-Up Studies Gene Expression Regulation, Bacterial Gene Regulatory Networks Genes, Bacterial Genome, Bacterial Host-Pathogen Interactions Humans Infant Male Molecular Sequence Data Mutation Phylogeny Polymorphism, Single Nucleotide Pseudomonas Infections Pseudomonas aeruginosa Respiratory System Signal Transduction Sputum Virulence Young Adult
