Journal article
Molecular characterization of serotype Asia-1 foot-and-mouth disease viruses in Pakistan and Afghanistan; emergence of a new genetic Group and evidence for a novel recombinant virus
National Veterinary Institute, Technical University of Denmark1
Food and Agriculture Organization of the United Nations2
Quaid-I-Azam University3
Sektion for Eksotiske Virussygdomme, Division of Virology, National Veterinary Institute, Technical University of Denmark4
Division of Virology, National Veterinary Institute, Technical University of Denmark5
Foot-and-mouth disease (FMD) is endemic in Pakistan and Afghanistan. The FMD virus serotypes O, A and Asia-1 are responsible for the outbreaks in these countries. Diverse strains of FMDV, even within the same serotype, co-circulate. Characterization of the viruses in circulation can facilitate appropriate vaccine selection and tracing of outbreaks.The present study characterized foot-and-mouth disease serotype Asia-1 viruses circulating in Pakistan and Afghanistan during the period 1998–2009.
Phylogenetic analysis of FMDV type Asia-1 revealed that three different genetic Groups of serotype Asia-1 have circulated in Pakistan during this time. These are Group-II, -VI and, recently, a novel Group (designated here as Group-VII). This new Group has not been detected in neighbouring Afghanistan during the study period but viruses from Groups I and -II are in circulation there.
Using near complete genome sequences, from FMD viruses of serotypes Asia-1 and A that are currently circulating in Pakistan, we have identified an interserotypic recombinant virus, which has the VP2-VP3-VP1-2A coding sequences derived from a Group-VII Asia-1 virus and the remainder of the genome from a serotype A virus of the A-Iran05AFG-07 sub-lineage.
The Asia-1 FMDVs currently circulating in Pakistan and Afghanistan are not efficiently neutralized by antisera raised against the Asia-1/Shamir vaccine strain. Thus, new Asia-1 vaccine strains may be required to block the spread of the current Asia-1 viruses.
Language: | English |
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Year: | 2011 |
Pages: | 2049-2062 |
ISSN: | 15677257 and 15671348 |
Types: | Journal article |
DOI: | 10.1016/j.meegid.2011.09.015 |
ORCIDs: | Belsham, Graham |
Afghanistan Afghanistan/epidemiology Amino Acid Sequence Animals Base Sequence Foot-and-Mouth Disease Foot-and-Mouth Disease Virus Foot-and-Mouth Disease Virus/classification Foot-and-Mouth Disease/epidemiology Likelihood Functions Molecular Sequence Data Pakistan Pakistan/epidemiology Phylogeny Recombination, Genetic Sequence Alignment Sequence Analysis, RNA Serotyping Viral Vaccines