Journal article
Synthesis of C-Glucosylated Octaketide Anthraquinones in Nicotiana benthamiana by Using a Multispecies-Based Biosynthetic Pathway
University of Copenhagen1
Chr. Hansen AS2
Department of Biotechnology and Biomedicine, Technical University of Denmark3
Eukaryotic Molecular Cell Biology, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark4
Biosynthetic Pathway Engineering, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark5
Carminic acid is a C-glucosylated octaketide anthraquinone and the main constituent of the natural dye carmine (E120), possessing unique coloring, stability, and solubility properties. Despite being used since ancient times, longstanding efforts to elucidate its route of biosynthesis have been unsuccessful.
Herein, a novel combination of enzymes derived from a plant (Aloe arborescens, Aa), a bacterium (Streptomyces sp. R1128, St), and an insect (Dactylopius coccus, Dc) that allows for the biosynthesis of the C-glucosylated anthraquinone, dcII, a precursor for carminic acid, is reported. The pathway, which consists of AaOKS, StZhuI, StZhuJ, and DcUGT2, presents an alternative biosynthetic approach for the production of polyketides by using a type III polyketide synthase (PKS) and tailoring enzymes originating from a type II PKS system.
The current study showcases the power of using transient expression in Nicotiana benthamiana for efficient and rapid identification of functional biosynthetic pathways, including both soluble and membrane-bound enzymes.
Language: | English |
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Year: | 2017 |
Pages: | 1893-1897 |
ISSN: | 14397633 and 14394227 |
Types: | Journal article |
DOI: | 10.1002/cbic.201700331 |
ORCIDs: | Mortensen, Uffe Hasbro , Frandsen, Rasmus John Normand , 0000-0002-6597-3431 , 0000-0003-4487-7886 , 0000-0003-0074-298X and 0000-0002-3252-3119 |