Journal article
Imaging of gadolinium spatial distribution in tumor tissue by laser ablation inductively coupled plasma mass spectrometry
Imaging Sciences Department, Metabolic and Molecular Imaging Group, MRC Clinical Sciences Center, Hammersmith Hospital, Imperial College London, London W120NN, UK.1
Laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) was utilized in postmortem imaging of gadolinium (Gd) spatial distribution in a mouse tumor model postadministration of PEGylated Gd liposomal nanoparticles. PEGylated liposomal nanoparticles were formulated using a paramagnetic lipid incorporating Gd, in addition to a fluorescent lipid, and injected intravenously into Balb/C nude mice bearing IGROV-1 tumors.
At postinjection (2 h), the tumors and selective organs were imaged by magnetic resonance imaging (MRI) and, after excision, by histology and LA-ICP-MS. The presence of Gd within tumor tissue was confirmed by LA-ICP-MS and when correlated to histology was found to be prevalent in regions of higher vascularity.
The presence of Gd in the kidneys was also confirmed. We have demonstrated, in a novel manner, the use of LA-ICP-MS for the spatial detection of Gd in tumor tissue. LA-ICP-MS is valuable in providing spatio-specific information of MRI contrast agents and more importantly Gd in tumor tissue.
| Language: | English |
|---|---|
| Publisher: | Springer-Verlag |
| Year: | 2010 |
| Pages: | 361-366 |
| ISSN: | 18602002 and 15361632 |
| Types: | Journal article |
| DOI: | 10.1007/s11307-009-0282-4 |
Animals Cell Line, Tumor Diagnostic Imaging Female Fluorescence imaging Gadolinium Gd biodistribution Gd contrast agents Humans Imaging / Radiology Kidney LA–ICP–MS Laser Therapy Liposomes Magnetic Resonance Imaging Medicine & Public Health Mice Mice, Inbred BALB C Microscopy, Fluorescence Multimodal imaging Nanoparticles Neoplasms Spectrophotometry, Atomic Tumor MRI
