Journal article
An in vitro methodology for discovering broadly-neutralizing monoclonal antibodies
Department of Biotechnology and Biomedicine, Technical University of Denmark1
Disease Systems Immunology, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark2
Tropical Pharmacology and Biotherapeutics, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark3
Technical University of Denmark4
DTU Proteomics Core, Section for Protein Science and Biotherapeutics, Department of Biotechnology and Biomedicine, Technical University of Denmark5
Eşkisehir Osmangazi University6
Broadly-neutralizing monoclonal antibodies are of high therapeutic utility against infectious diseases caused by bacteria and viruses, as well as different types of intoxications. Snakebite envenoming is one such debilitating pathology, which is currently treated with polyclonal antibodies derived from immunized animals.
For the development of novel envenoming therapies based on monoclonal antibodies with improved therapeutic benefits, new discovery approaches for broadly-neutralizing antibodies are needed. Here, we present a methodology based on phage display technology and a cross-panning strategy that enables the selection of cross-reactive monoclonal antibodies that can broadly neutralize toxins from different snake species.
This simple in vitro methodology is immediately useful for the development of broadly-neutralizing (polyvalent) recombinant antivenoms with broad species coverage, but may also find application in the development of broadly-neutralizing antibodies against bacterial, viral, and parasitic agents that are known for evading therapy via resistance mechanisms and antigen variation.
| Language: | English |
|---|---|
| Publisher: | Nature Publishing Group UK |
| Year: | 2020 |
| Pages: | 10765 |
| ISSN: | 20452322 |
| Types: | Journal article |
| DOI: | 10.1038/s41598-020-67654-7 |
| ORCIDs: | Ahmadi, Shirin , Jürgensen, Jonas A. , Ledsgaard, Line , Schoof, Erwin M. , Sørensen, Christoffer V. and Laustsen, Andreas H. |
Animals Antibodies, Monoclonal Antibodies, Neutralizing Antigens Antivenins Biotechnology Cross Reactions Drug Design Drug discovery Enzyme-Linked Immunosorbent Assay Humans Immunoglobulin Fab Fragments In Vitro Techniques Inhibitory Concentration 50 Keratinocytes Mass Spectrometry Neutralization Tests Peptide Library Proteomics Recombinant Proteins Snakes Venoms
