Journal article
Accumulation of Nano-sized Particles in a Murine Model of Angiogenesis
Department of Experimental Clinical Oncology, Aarhus University Hospital, Noerrebrogade 44, 8000 Aarhus C, Denmark1
Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark2
Department of Molecular Biology and Genetics, Aarhus University, C.F. Moellers Allé 3, 8000 Aarhus C, Denmark3
The Lundbeck Foundation Nanomedicine Center for Individualized Management of Tissue Damage and Regeneration (LUNA), Aarhus University, Aarhus, Denmark4
Center of Functionally Integrative Neuroscience (CFIN), Aarhus University Hospital, Noerrebrogade 44, 8000 Aarhus C, Denmark5
Department of Engineering, Aarhus University, Finlandsgade 22, 8000 Aarhus, Denmark6
Department of Biomedicine, Aarhus University, Bartholins Allé 6, 8000 Aarhus, Denmark7
Center for Insoluble Protein Structures (inSPIN) and Department of Chemistry, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark8
To evaluate the ability of nm-scaled iron oxide particles conjugated with Azure A, a classic histological dye, to accumulate in areas of angiogenesis in a recently developed murine angiogenesis model. We characterized the Azure A particles with regard to their hydrodynamic size, zeta potential, and blood circulation half-life.
The particles were then investigated by Magnetic Resonance Imaging (MRI) in a recently developed murine angiogenesis model along with reference particles (Ferumoxtran-10) and saline injections. The Azure A particles had a mean hydrodynamic diameter of 51.8 ± 43.2 nm, a zeta potential of -17.2 ± 2.8 mV, and a blood circulation half-life of 127.8 ± 74.7 minutes.
Comparison of MR images taken pre- and 24-hours post- injection revealed a significant increase in R2* relaxation rates for both Azure A and Ferumoxtran-10 particles. No significant difference was found for the saline injections. The relative increase was calculated for the three groups, and showed a significant difference between the saline group and the Azure A group, and between the saline group and the Ferumoxtran-10 group.
However, no significant difference was found between the two particle groups. Ultrahigh-field MRI revealed localization of both types of iron oxide particles to areas of neovasculature. However, the Azure A particles did not show any enhanced accumulation relative to Ferumoxtran-10, suggesting the accumulation in both cases to be passive.
| Language: | Undetermined |
|---|---|
| Publisher: | Elsevier BV |
| Year: | 2015 |
| Pages: | 470-476 |
| ISSN: | 10902104 and 0006291x |
| Types: | Journal article |
| DOI: | 10.1016/j.bbrc.2013.11.127 |
| ORCIDs: | Nygaard, Jens V. , Kjems, Jørgen and Nielsen, Niels Chr. |
