Journal article
The gold-standard genome of Aspergillus niger NRRL 3 enables a detailed view of the diversity of sugar catabolism in fungi
Westerdijk Fungal Biodiversity Institute1
Department of Biotechnology and Biomedicine, Technical University of Denmark2
Concordia University3
Joint Genome Institute4
Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark5
Network Engineering of Eukaryotic Cell factories, Section for Synthetic Biology, Department of Biotechnology and Biomedicine, Technical University of Denmark6
Utrecht University7
The fungal kingdom is too large to be discovered exclusively by classical genetics. The access to omics data opens a new opportunity to study the diversity within the fungal kingdom and how adaptation to new environments shapes fungal metabolism. Genomes are the foundation of modern science but their quality is crucial when analysing omics data.
In this study, we demonstrate how one gold-standard genome can improve functional prediction across closely related species to be able to identify key enzymes, reactions and pathways with the focus on primary carbon metabolism. Based on this approach we identified alternative genes encoding various steps of the different sugar catabolic pathways, and as such provided leads for functional studies into this topic.
We also revealed significant diversity with respect to genome content, although this did not always correlate to the ability of the species to use the corresponding sugar as a carbon source.
Language: | English |
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Publisher: | CBS Fungal Biodiversity Centre |
Year: | 2018 |
Pages: | 61-78 |
ISSN: | 18729797 and 01660616 |
Types: | Journal article |
DOI: | 10.1016/j.simyco.2018.10.001 |
ORCIDs: | Brandl, J. , Nybo, J.L. , Vesth, T.C. and Andersen, M.R. |