Journal article
Genomic relationships of Actinobacillus pleuropneumoniae serotype 2 strains evaluated by ribotyping, sequence analysis of ribosomal intergenic regions, and pulsed-field gel electrophoresis
The aim of the present study was to examine the genomic relationship among 112 Actinobacillus pleuropneumoniae serotype 2 strains obtained throughout Europe and North America. HindIII ribotyping of the strains resulted in five ribotypes of high similarity (87-98%). Sequence analysis of the ribosomal intergenic region of strains representing each ribotype and each country showed no differences.
A common ribotype was further characterized by PFGE of 12 strains representing all countries. The resultant five PFGE patterns of European strains showed a similarity of more than 91%, to which the two PFGE patterns of the Canadian strains showed a similarity of 84-86%. In conclusion, strains of A, pleuropneumoniae serotype 2 seem to be clonally related.
| Language: | English |
|---|---|
| Year: | 1998 |
| Pages: | 211-215 |
| ISSN: | 13645072 , 02668254 and 1472765x |
| Types: | Journal article |
| DOI: | 10.1046/j.1472-765X.1998.t01-1-00419.x |
Actinobacillus Infections Actinobacillus pleuropneumoniae Animals Bacterial Typing Techniques Canada Cluster Analysis Deoxyribonucleases, Type II Site-Specific Electrophoresis, Gel, Pulsed-Field Europe Genes, rRNA Lung RNA, Ribosomal, 16S RNA, Ribosomal, 23S Restriction Mapping Sequence Analysis, DNA Serotyping Swine Swine Diseases
