Journal article
The interplay between sulphur and selenium metabolism influences the intracellular redox balance in Saccharomyces cerevisiae
Selenium (Se) is an essential element for most eukaryotic organisms, including humans. The balance between Se toxicity and its beneficial effects is very delicate. It has been demonstrated that a diet enriched with Se has cancer prevention potential in humans. The most popular commercial Se supplementation is selenized yeast, which is produced in a fermentation process using an inorganic source of Se.
Here, we show that the uptake of Se, Se toxic effects and intracellular Se-metabolite profile are largely influenced by the level of sulphur source supplied during the fermentation. A Yap1-dependent oxidative stress response is active when yeast actively metabolizes Se, and this response is linked to the generation of intracellular redox imbalance.
The redox imbalance derives from a disproportionate ratio between the reduced and oxidized forms of glutathione and also from the influence of Se metabolism on the central carbon metabolism. The observed increase in glycerol production rate, concomitant with the inhibition of ethanol formation in the presence of Se, can be ascribed to the occurrence of redox imbalance that triggers glycerol biosynthesis to replenish the pool of NAD+.
Language: | English |
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Year: | 2012 |
Pages: | 20-32 |
ISSN: | 15671364 and 15671356 |
Types: | Journal article |
DOI: | 10.1111/j.1567-1364.2011.00757.x |
10060, Biochemistry studies - General 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines 10069, Biochemistry studies - Minerals 13002, Metabolism - General metabolism and metabolic pathways 22501, Toxicology - General and methods 51519, Plant physiology - Metabolism Fungi Plantae (Fungi, Microorganisms, Nonvascular Plants, Plants) - Ascomycetes [15100] Saccharomyces cerevisiae species strain-MATa, strain-pRSGFP-YAP1, strain-MATa ura3-52, strain-MAL2-8C, strain-SUC2, strain-CEN.PK113-5D, strain-CEN.PK113-7D Metabolism NAD 53-84-9 SDG 3 - Good Health and Well-being Toxicology Yap1 carbon 7440-44-0 metabolism ethanol 64-17-5 glycerol 56-81-5 biosynthesis production intracellular redox balance oxidative stress response oxidized glutathione 27025-41-8 selenium 7782-49-2 toxin metabolism sulfur 7704-34-9 metabolism