Journal article
RT-PCR-ELISA as a tool for diagnosis of low-pathogenicity avian influenza
National Veterinary Institute1
DNA Technology A/S2
Section of Poultry Diseases, Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark3
Division of Poultry, Fish and Fur Animals, National Veterinary Institute, Technical University of Denmark4
National Veterinary Institute, Technical University of Denmark5
A one-tube reverse transcriptase/polymerase chain reaction coupled with an enzyme-linked immunosorbent assay (RT-PCR-ELISA) was developed for the rapid detection of avian influenza virus (AIV) in clinical specimens. A total of 419 swab pools were analyzed from chickens experimentally infected with low-pathogenicity AIV, from wild aquatic birds, and from domestic ducks.
The AIV was detected in 32 swab pools by RT-PCR-ELISA compared to 23 by virus isolation (VI) in embryonated specific pathogen free (SPF) chicken eggs. Thus, 39% more specimens were positive by RT-PCR-ELISA than by VI. Two of the twenty-three VI-positive specimens were negative when tested by RT-PCR-ELISA.
The diagnostic sensitivity and specificity of the RT-PCR-ELISA was 91% and 97%, respectively, using VI in SPF eggs as the gold reference standard.
| Language: | English |
|---|---|
| Publisher: | American Association of Avian Pathologists, Inc. |
| Year: | 2003 |
| Pages: | 1075-1078 |
| ISSN: | 19384351 and 00052086 |
| Types: | Journal article |
| DOI: | 10.1637/0005-2086-47.s3.1075 |
Allantois Animals Base Sequence Birds Chick Embryo DNA Primers Enzyme-Linked Immunosorbent Assay Hemagglutinin Glycoproteins, Influenza Virus Influenza A virus Influenza in Birds Method evaluation Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Self-Sustained Sequence Replication
