Journal article
Metabolite secretion in microorganisms: the theory of metabolic overflow put to the test
The New Zealand Institute for Plant and Food Research Limited1
The University of Auckland2
University of Minho3
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark4
Yeast Cell Factories, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark5
Introduction Microbial cells secrete many metabolites during growth, including important intermediates of the central carbon metabolism. This has not been taken into account by researchers when modeling microbial metabolism for metabolic engineering and systems biology studies. Materials and Methods The uptake of metabolites by microorganisms is well studied, but our knowledge of how and why they secrete different intracellular compounds is poor.
The secretion of metabolites by microbial cells has traditionally been regarded as a consequence of intracellular metabolic overflow. Conclusions Here, we provide evidence based on time-series metabolomics data that microbial cells eliminate some metabolites in response to environmental cues, independent of metabolic overflow.
Moreover, we review the different mechanisms of metabolite secretion and explore how this knowledge can benefit metabolic modeling and engineering.
Language: | English |
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Publisher: | Springer US |
Year: | 2018 |
Pages: | 43 |
Journal subtitle: | An Official Journal of the Metabolomics Society |
ISSN: | 15733890 and 15733882 |
Types: | Journal article |
DOI: | 10.1007/s11306-018-1339-7 |