Journal article
High-throughput label-free detection of Ochratoxin A in wine using supported liquid membrane extraction and Ag-capped silicon nanopillar SERS substrates
Technical University of Denmark1
Nanoprobes, Drug Delivery and Sensing, Department of Health Technology, Technical University of Denmark2
Drug Delivery and Sensing, Department of Health Technology, Technical University of Denmark3
Department of Health Technology, Technical University of Denmark4
Center for Intelligent Drug Delivery and Sensing Using Microcontainers and Nanomechanics, Department of Health Technology, Technical University of Denmark5
Research Group for Analytical Food Chemistry, National Food Institute, Technical University of Denmark6
National Food Institute, Technical University of Denmark7
Iranian National Institute for Oceanography and Atmospheric Science8
Mycotoxins are toxic secondary metabolites produced by fungi, found in a variety of food sources and cause diseases and death in both humans and animals. On-site and easy to use detection would be significant improvement considering the currently used methods. We present an approach, based on surface-enhanced Raman spectroscopy (SERS), where label-free detection of Ochratoxin A (OTA) was achieved in a multiwell SERS detection platform.
When quantifying OTA in wine samples the SERS-based detection was used in combination with high throughput supported liquid membrane (SLM) extraction. The obtained detection limit in white wine was 115 ppb and we found that the SERS-based quantification is comparable with LC-MS. However, we also found that the matrix effect from red wine was interfering with both SERS and LC-MS detection.
The SLM-SERS method provides a time and cost effective approach for OTA detection, which is a clear advantage over other extraction and direct detection approaches.
Language: | English |
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Year: | 2020 |
Pages: | 107183 |
ISSN: | 18737129 and 09567135 |
Types: | Journal article |
DOI: | 10.1016/j.foodcont.2020.107183 |
ORCIDs: | Zor, Kinga , Zhai, Demi Shuang , Viehrig, Marlitt , Smedsgaard, Jørn , Rindzevicius, Tomas and Boisen, Anja |