Journal article
Sequence specific inhibition of DNA restriction enzyme cleavage by PNA
Plasmids containing double-stranded 10-mer PNA (peptide nucleic acid chimera) targets proximally flanked by two restriction enzyme sites were challenged with the complementary PNA or PNAs having one or two mismatches, and the effect on the restriction enzyme cleavage of the flanking sites was assayed.
The following PNAs were used: T10-LysNH2, T5CT4-LysNH2 and T2CT2CT4-LysNH2 and the corresponding targets cloned into pUC 19 were flanked by BamH1, Sal1 or Pstl sites, respectively. In all cases it was found that complete inhibition of restriction enzyme cleavage was obtained with the complementary PNA, a significantly reduced effect was seen with a PNA having one mismatch, and no effect was seen with a PNA having two mismatches.
These results show that PNA can be used as sequence specific blockers of DNA recognizing proteins.
| Language: | English |
|---|---|
| Year: | 1993 |
| Pages: | 197-200 |
| ISSN: | 13624962 and 03051048 |
| Types: | Journal article |
| DOI: | 10.1093/nar/21.2.197 |
| ORCIDs: | 0000-0001-8771-330X |
Base Sequence CTGCAG-specific type II deoxyribonucleases Chimera DNA DNA Restriction Enzymes DNA Restriction Enzymes/antagonists & inhibitors Deoxyribonuclease BamHI Deoxyribonuclease BamHI/antagonists & inhibitors Deoxyribonucleases, Type II Site-Specific Deoxyribonucleases, Type II Site-Specific/antagonists & inhibitors GTCGAC-specific type II deoxyribonucleases Molecular Sequence Data Oligodeoxyribonucleotides Oligodeoxyribonucleotides/metabolism Peptides Peptides/metabolism Restriction Mapping
