Journal article
Polysaccharides serve as scaffold of biofilms formed by mucoid Pseudomonas aeruginosa
Department of Systems Biology, Technical University of Denmark1
Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark2
Copenhagen University Hospital Herlev and Gentofte3
Technical University of Denmark4
University of Copenhagen5
Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark6
Bacterial Cell Factories, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark7
Chronic lung infection by mucoid Pseudomonas aeruginosa is one of the major pathologic features in patients with cystic fibrosis. Mucoid P. aeruginosa is notorious for its biofilm forming capability and resistance to immune attacks. In this study, the roles of extracellular polymeric substances from biofilms formed by mucoid P. aeruginosa were investigated.
Alginate is not an essential structure component for mucoid P. aeruginosa biofilms. Genetic studies revealed that Pel and Psl polysaccharides serve as essential scaffold and mediate macrocolony formation in mucoid P. aeruginosa biofilms. The Psl polysaccharide is more important than Pel polysaccharide in mucoid P. aeruginosa biofilm structure maintenance and phagocytosis resistance.
The polysaccharides were further found to protect mucoid P. aeruginosa strain from host immune clearance in a mouse model of acute lung infection.
| Language: | English |
|---|---|
| Year: | 2012 |
| Pages: | 366-376 |
| ISSN: | 1574695x and 09288244 |
| Types: | Journal article |
| DOI: | 10.1111/j.1574-695X.2012.00936.x |
| ORCIDs: | Molin, Søren , 0000-0001-5004-8609 and 0000-0002-1347-725X |
