Journal article
Modular microfluidic system as a model of cystic fibrosis airways
Department of Micro- and Nanotechnology, Technical University of Denmark1
Fluidic Array Systems and Technology, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Systems Biology, Technical University of Denmark3
Center for Systems Microbiology, Department of Systems Biology, Technical University of Denmark4
University College Cork5
Polymer Micro & Nano Engineering, Department of Micro- and Nanotechnology, Technical University of Denmark6
Bioanalytics, Department of Micro- and Nanotechnology, Technical University of Denmark7
A modular microfluidic airways model system that can simulate the changes in oxygen tension in different compartments of the cystic fibrosis (CF) airways was designed, developed, and tested. The fully reconfigurable system composed of modules with different functionalities: multichannel peristaltic pumps, bubble traps, gas exchange chip, and cell culture chambers.
We have successfully applied this system for studying the antibiotic therapy of Pseudomonas aeruginosa, the bacteria mainly responsible for morbidity and mortality in cystic fibrosis, in different oxygen environments. Furthermore, we have mimicked the bacterial reinoculation of the aerobic compartments (lower respiratory tract) from the anaerobic compartments (cystic fibrosis sinuses) following an antibiotic treatment.
This effect is hypothesised as the one on the main reasons for recurrent lung infections in cystic fibrosis patients.
| Language: | English |
|---|---|
| Publisher: | American Institute of Physics |
| Year: | 2012 |
| Pages: | 34109 |
| ISSN: | 19321058 |
| Types: | Journal article |
| DOI: | 10.1063/1.4742911 |
| ORCIDs: | Molin, Søren , Taboryski, Rafael J. , Sternberg, Claus , Dufva, Martin and Emnéus, Jenny |
