Conference paper
Full genome sequence of a Danish isolate of Mycobacterium avium subspecies paratuberculosis, strain Ejlskov2007
Technical University of Denmark1
Adaptive Immunology & Parasitology, Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark2
Division of Veterinary Diagnostics and Research, National Veterinary Institute, Technical University of Denmark3
National Veterinary Institute, Technical University of Denmark4
Center for Biological Sequence Analysis, Department of Systems Biology, Technical University of Denmark5
Department of Systems Biology, Technical University of Denmark6
We have sequenced a Danish isolate of Mycobacterium avium subspecies paratuberculosis, strain Ejlskov2007. The strain was isolated from faecal material of a 48 month old second parity Danish Holstein cow, with clinical symptoms of chronic diarrhoea and emaciation. The cultures were grown on Löwenstein-Jensen media by standard procedures and passed once to new tubes before DNA extraction and being sequenced on a 454 FLX machine.
Currently, the genome has been assembled into 70 contiguous pieces, for a total of around 5.0 Mbp, with a 63% GC content. We have predicted a total of 4687 proteins, consisting of 4317 unique gene families. Comparison with M. avium paratuberculosis strain K10 revealed only 3436 genes in common (~70%).
We have used GenomeAtlases to show conserved (and unique) regions along the Ejlskov2007 chromosome, compared to 2 other Mycobacterium avium sequenced genomes. Pan-genome analyses of the sequenced Mycobacterium genomes reveal a surprisingly open and diverse set of genes for this bacterial genera.
| Language: | English |
|---|---|
| Year: | 2009 |
| Proceedings: | 10th International Colloquium on Paratuberculosis |
| Types: | Conference paper |
| ORCIDs: | Mikkelsen, Heidi , Lund, Ole , Nielsen, Morten and Jungersen, Gregers |
