Journal article
Identification of Differentially Expressed Gene Transcripts in Porcine Endometrium during Early Stages of Pregnancy
Polish Academy of Sciences1
Wageningen UR Livestock Research2
University of Life Sciences in Lublin3
Medical University of Gdańsk4
Nicolaus Copernicus University in Toruń5
Wroclaw University of Environmental and Life Sciences6
University of Warmia and Mazury in Olsztyn7
Department of Applied Mathematics and Computer Science, Technical University of Denmark8
Statistics and Data Analysis, Department of Applied Mathematics and Computer Science, Technical University of Denmark9
During the early stages of pregnancy, the uterine endometrium undergoes dramatic morphologic and functional changes accompanied with dynamic variation in gene expression. Pregnancy-stage specific differentially expressed gene (DEG)-transcript-probes were investigated and identified by comparing endometrium transcriptome at 9th day (9D), 12th day (12D) and 16th day (16D) of early pregnancy in Polish large-white (PLW) gilts.
Endometrium comparisons between 9D-vs-12D, 9D-vs-16D and 12D-vs-16D of early pregnancy identified 6049, 374 and 6034 highly significant DEG-transcript-probes (p <0.001; >2 FC). GO term enrichment analysis identified commonly shared upregulated endometrial DEG-transcript-probes (p <0.001; >2 FC), that were regulating the gene functions of anatomic structure development and transport (TG), DNA-binding and methyltransferase activity (ZBTB2), ion-binding and kinase activity (CKM), cell proliferation and apoptosis activity (IL1B).
Downregulated DEG-transcript-probes (p <0.001; >2 FC) were involved in regulating the gene functions of phosphatase activity (PTPN11), TC616413 gene-transcript and Sus-scrofa LOC100525539. Moreover, blastn comparison of microarray-probes sequences against sus-scrofa11 assembly identified commonly shared upregulated endometrial DEG-transcript-probes (E <0.06; >2 FC), that were regulating the gene functions of reproduction and growth (SELENOP), cytoskeleton organization and kinase activity (CDC42BPA), phosphatase activity (MINPP1), enzyme-binding and cell-population proliferation (VAV3), cancer-susceptibility candidate gene (CASC4), cytoskeletal protein-binding (COBLL1), ion-binding, enzyme regulator activity (ACAP2) Downregulated endometrial DEG-transcript-probes (E <0.06; >2FC) were involved in regulating the gene functions of signal-transduction (TMEM33), catabolic and metabolic processes (KLHL15).
Microarray validation experiment on selected candidate genes showed complementarity to significant endometrial DEG-transcript-probes responsible for the regulation of immune response (IL1B, S100A11), lipid metabolism (FABP3, PPARG), cell-adhesion (ITGAV), angiogenesis (IL1B), intercellular transmission (NMB), cell-adhesion (OPN) and response to stimuli (RBP4) was confirmed by RT-PCR.
This study provides a clue that identified pregnancy-stage specific microarray transcript probes could be considered as candidate genes for recognition and establishment of early pregnancy in the pig.
Language: | English |
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Publisher: | MDPI |
Year: | 2020 |
Pages: | 68 |
ISSN: | 20751729 |
Types: | Journal article |
DOI: | 10.3390/life10050068 |
ORCIDs: | 0000-0001-6226-4114 , 0000-0003-2139-8241 , 0000-0002-2997-9991 , N. Kadarmideen, Haja , 0000-0002-0329-787X , 0000-0001-7001-1336 , 0000-0002-0937-2586 , 0000-0002-6097-9826 , 0000-0002-6362-5425 , 0000-0003-4585-0781 , 0000-0001-8849-3011 , 0000-0003-3933-2400 , 0000-0003-1401-2558 , 0000-0002-4676-7990 , 0000-0003-1901-6101 , 0000-0002-2279-9234 , 0000-0002-7346-547X , 0000-0003-1056-0784 and 0000-0002-9065-2197 |