Journal article
Large scale biomimetic membrane arrays
Bioanalytics Group, Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark1
Biomedical Micro Systems Section, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Aquaporin A/S4
Polymeric Enabling Microsystems Group, Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark5
Polymer Micro and Nano Engineering Section, Department of Micro- and Nanotechnology, Technical University of Denmark6
Biophysics and Fluids, Department of Physics, Technical University of Denmark7
Department of Physics, Technical University of Denmark8
To establish planar biomimetic membranes across large scale partition aperture arrays, we created a disposable single-use horizontal chamber design that supports combined optical-electrical measurements. Functional lipid bilayers could easily and efficiently be established across CO2 laser micro-structured 8 x 8 aperture partition arrays with average aperture diameters of 301 +/- 5 mu m.
We addressed the electro-physical properties of the lipid bilayers established across the micro-structured scaffold arrays by controllable reconstitution of biotechnological and physiological relevant membrane peptides and proteins. Next, we tested the scalability of the biomimetic membrane design by establishing lipid bilayers in rectangular 24 x 24 and hexagonal 24 x 27 aperture arrays, respectively.
The results presented show that the design is suitable for further developments of sensitive biosensor assays, and furthermore demonstrate that the design can conveniently be scaled up to support planar lipid bilayers in large square-centimeter partition arrays.
| Language: | English |
|---|---|
| Publisher: | Springer-Verlag |
| Year: | 2009 |
| Pages: | 719-727 |
| ISSN: | 16182650 and 16182642 |
| Types: | Journal article |
| DOI: | 10.1007/s00216-009-3010-7 |
| ORCIDs: | Emnéus, Jenny and Helix Nielsen, Claus |
DERIVATIVES FUSOBACTERIUM-NUCLEATUM GRAMICIDIN IN-SITU ION CHANNELS LIPID-BILAYER-MEMBRANES MOLECULES PROTEIN REMOTE PLASMAS SURFACE MODIFICATION
Analytical Chemistry Array Bacillus Bacterial Outer Membrane Proteins Bacterial Toxins Biochemistry, general Biomimetics Black lipid membrane Chemistry Electrochemical Techniques Environmental Monitoring/Analysis Equipment Design Food Science Gramicidin Hemolysin Proteins Laboratory Medicine Lipid Bilayers Measurement Science and Instrumentation Membrane Proteins Membrane protein Membrane-spanning peptides Membranes, Artificial Optical–electrical measurements Peptides, Cyclic Staphylococcus aureus Valinomycin fomA protein, Fusobacterium staphylococcal alpha-toxin
