Journal article
Multichannel Bipotentiostat Integrated With a Microfluidic Platform for Electrochemical Real-Time Monitoring of Cell Cultures
Polytechnic University of Milan1
University of Genoa2
Department of Micro- and Nanotechnology, Technical University of Denmark3
Bioanalytics, Department of Micro- and Nanotechnology, Technical University of Denmark4
University of Potsdam5
Fluidic Array Systems and Technology, Department of Micro- and Nanotechnology, Technical University of Denmark6
Nano Bio Integrated Systems, Department of Micro- and Nanotechnology, Technical University of Denmark7
An electrochemical detection system specifically designed for multi-parameter real-time monitoring of stem cell culturing/differentiation in a microfluidic system is presented. It is composed of a very compact 24-channel electronic board, compatible with arrays of microelectrodes and coupled to a microfluidic cell culture system.
A versatile data acquisition software enables performing amperometry, cyclic voltammetry and impedance spectroscopy in each of the 12 independent chambers over a 100 kHz bandwidth with current resolution down to 5 pA for 100 ms measuring time. The design of the platform, its realization and experimental characterization are reported, with emphasis on the analysis of impact of input capacitance (i.e., microelectrode size) and microfluidic pump operation on current noise.
Programmable sequences of successive injections of analytes (ferricyanide and dopamine) and rinsing buffer solution as well as the impedimetric continuous tracking for seven days of the proliferation of a colony of PC12 cells are successfully demonstrated.
| Language: | English |
|---|---|
| Publisher: | IEEE |
| Year: | 2012 |
| Pages: | 498-507 |
| ISSN: | 19409990 and 19324545 |
| Types: | Journal article |
| DOI: | 10.1109/TBCAS.2012.2187783 |
| ORCIDs: | Heiskanen, Arto , Zor, Kinga , Dufva, Martin , Dimaki, Maria and Emnéus, Jenny |
Animals Bandwidth Biomedical Engineering Biosensing Techniques Cell Culture Techniques Cell Proliferation Computer Systems Computer architecture Current measurement Dielectric Spectroscopy Dopamine Electrochemical Techniques Electrochemical measurements Electrodes Equipment Design Impedance Instruments Microfluidic Analytical Techniques Microprocessors PC12 Cells Potentiometry Rats Signal Processing, Computer-Assisted Software impedance spectroscopy microfluidics multichannel potentiostat stem cell monitoring
