Journal article
Revealing the Compact Structure of Lactic Acid Bacterial Heteroexopolysaccharides by SAXS and DLS
Department of Micro- and Nanotechnology, Technical University of Denmark1
Amphiphilic Polymers in Biological Sensing, Department of Micro- and Nanotechnology, Technical University of Denmark2
Department of Biotechnology and Biomedicine, Technical University of Denmark3
Enzyme and Protein Chemistry, Section for Protein Chemistry and Enzyme Technology, Department of Biotechnology and Biomedicine, Technical University of Denmark4
Department of Chemistry, Technical University of Denmark5
X-ray Crystallography, Department of Chemistry, Technical University of Denmark6
Agriculture and Agri-Food Canada7
University of Copenhagen8
Molecular structures of exopolysaccharides are required to understand their functions and the relationships between the structure and physical and rheological properties. Small-angle X-ray scattering and dynamic light scattering were used in conjunction with molecular modeling to characterize solution structures of three lactic acid bacterial hetero-exopolysaccharides (HePS-1, HePS-2 and HePS-3).
Values of radius of gyration RG, cross-sectional radius of gyration RXS, approximate length L and hydrodynamic diameter were not directly proportional to the molar mass and indicated the HePSs adopted a compact coil-like rather than an extended conformation. Constrained molecular modeling of 15,000 randomised HePS-1 conformers resulted in five best-fit structures with R factor of 3.94.6% revealing random coil-like structure. Φ and Ψ angle analysis of glycosidic linkages in HePS-1 structures suggests Galf residues significantly influence the conformation.
Ab initio scattering modeling of HePS-2 and HePS-3 gave excellent curve fittings with χ2 of 0.43 and 0.34 for best-fit models, respectively, compatible with coil-like conformation. The findings disclose solution behaviour of HePS relevant for their interactions with biomacromolecules e.g. milk proteins.
Language: | English |
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Publisher: | American Chemical Society (ACS) |
Year: | 2017 |
Pages: | 747-757 |
ISSN: | 15264602 and 15257797 |
Types: | Journal article |
DOI: | 10.1021/acs.biomac.6b01597 |
ORCIDs: | 0000-0003-2658-414X , Khan, Sanaullah , Almdal, Kristoffer , Harris, Pernille , Peters, Günther H.J. and Svensson, Birte |